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Cardiovascular Research 2000 48(1):168-177; doi:10.1016/S0008-6363(00)00174-7
© 2000 by European Society of Cardiology
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Copyright © 2000, European Society of Cardiology

Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress

Li-ming Gan, Lena Selin-Sjögren, Roya Doroudi and Sverker Jern*

Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Östra, Göteborg University, SE 416 85 Gothenburg, Sweden

* Corresponding author. Tel.: +46-31-343-5921; fax: +46-31-191-416 sverker.jern{at}hjl.gu.se

Objective: By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels. Methods: Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm2. ET-1 and eNOS gene and protein expression was quantified with real-time reverse-transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively. Results: Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system. Conclusion: Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone.

KEYWORDS Endothelial function; Mechanotransduction; Endothelins; Nitric oxide; Gene expression; Veins


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