Skip Navigation

Cardiovascular Research 2000 48(1):168-177; doi:10.1016/S0008-6363(00)00174-7
© 2000 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gan, L.-m.
Right arrow Articles by Jern, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gan, L.-m.
Right arrow Articles by Jern, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2000, European Society of Cardiology

Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress

Li-ming Gan, Lena Selin-Sjögren, Roya Doroudi and Sverker Jern*

Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Östra, Göteborg University, SE 416 85 Gothenburg, Sweden

* Corresponding author. Tel.: +46-31-343-5921; fax: +46-31-191-416 sverker.jern{at}hjl.gu.se

Objective: By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels. Methods: Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm2. ET-1 and eNOS gene and protein expression was quantified with real-time reverse-transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively. Results: Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system. Conclusion: Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone.

KEYWORDS Endothelial function; Mechanotransduction; Endothelins; Nitric oxide; Gene expression; Veins


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. Bien, A. Riad, C. A. Ritter, M. Gratz, F. Olshausen, D. Westermann, M. Grube, T. Krieg, S. Ciecholewski, S. B. Felix, et al.
The Endothelin Receptor Blocker Bosentan Inhibits Doxorubicin-Induced Cardiomyopathy
Cancer Res., November 1, 2007; 67(21): 10428 - 10435.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. C. Nystrom, P. Lindblom, A. Wickman, I. Andersson, J. Norlin, J. Faldt, P. Lindahl, O. Skott, M. Bjarnegard, S. M. Fitzgerald, et al.
Platelet-derived growth factor B retention is essential for development of normal structure and function of conduit vessels and capillaries
Cardiovasc Res, August 1, 2006; 71(3): 557 - 565.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. A. Mawji, G. B. Robb, S. C. Tai, and P. A. Marsden
Role of the 3'-Untranslated Region of Human Endothelin-1 in Vascular Endothelial Cells: CONTRIBUTION TO TRANSCRIPT LABILITY AND THE CELLULAR HEAT SHOCK RESPONSE
J. Biol. Chem., March 5, 2004; 279(10): 8655 - 8667.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. L. Fry
Arterial intimal-medial permeability and coevolving structural responses to defined shear-stress exposures
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2341 - H2355.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.