© 1999 by European Society of Cardiology
Copyright © 1999, European Society of Cardiology
Abnormal Ca2+ release from cardiac sarcoplasmic reticulum in tachycardia-induced heart failure
Second Department of Internal Medicine, Yamaguchi University School of Medicine, 1144 Kogushi, Ube, Yamaguchi, Japan
* Corresponding author. +81-836-22-2248; fax: +81-836-22-2246
Objective: In heart failure, little information is available as to the Ca2+ release function of sarcoplasmic reticulum (SR), which plays a major role in cardiac contractile function. Here, we assessed the rapid kinetics of drug-induced Ca2+ release from cardiac SR in combination with a measurement of ryanodine binding in heart failure. Methods: The SR vesicles were isolated from dog left ventricular (LV) muscles (normal (N), n=10; pacing induced heart failure (HF), n=10). The time course of SR Ca2+ release was continuously monitored by a stopped-flow apparatus using arsenazoIII as a Ca2+ indicator, and Ca2+ uptake and [3H]ryanodine binding assays were done using a filtration method. Results: The amount of Ca2+ uptake was reduced in HF to 55% of N (P<0.05). Even the more marked and earlier appeared decrease was seen in the rate constant and the initial rate of polylysine (PL; a specific release trigger)-induced Ca2+ release (P<0.05). However, the PL concentration dependency of the initial rate shifted towards lower concentrations of PL in HF than in N ([PL] at half maximum stimulation=0.13 vs. 0.35 µM). The [3H]ryanodine binding assay revealed a lower Bmax (pmol/mg) in HF than in N (0.91±0.19 vs. 2.64±0.59, P<0.05), but no difference in Kd (nM) (0.95±0.29 vs. 0.90±0.11, P=n.s.). The [PL] dependency on the enhancement of [3H]ryanodine binding again showed a shift towards lower [PL] in HF than in N. Conclusions: In pacing-induced heart failure, the Ca2+ releasing function of SR is disturbed, which may result in an intra-cellular Ca2+ transient that was slowed down.
KEYWORDS SR, sarcoplasmic reticulum; E–C coupling, excitation–contraction coupling; PMSF, phenylmethanesulfonyl fluoride; MES, 2-(N-morpholino)ethanesulfonic acid; MOPS, 3-[N-morpholino]propanesulfonic acid; EGTA, ethylene glycol bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Lehnart and A. R. Marks Regulation of Ryanodine Receptors in the Heart Circ. Res., October 12, 2007; 101(8): 746 - 749. [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Lehnart, X. H.T. Wehrens, and A. R. Marks Defective Ryanodine Receptor Interdomain Interactions May Contribute to Intracellular Ca2+ Leak: A Novel Therapeutic Target in Heart Failure Circulation, June 28, 2005; 111(25): 3342 - 3346. [Full Text] [PDF]
|
|
|
|
|
|
T. Oda, M. Yano, T. Yamamoto, T. Tokuhisa, S. Okuda, M. Doi, T. Ohkusa, Y. Ikeda, S. Kobayashi, N. Ikemoto, et al. Defective Regulation of Interdomain Interactions Within the Ryanodine Receptor Plays a Key Role in the Pathogenesis of Heart Failure Circulation, June 28, 2005; 111(25): 3400 - 3410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nerheim, S. Birger-Botkin, L. Piracha, and B. Olshansky Heart Failure and Sudden Death in Patients With Tachycardia-Induced Cardiomyopathy and Recurrent Tachycardia Circulation, July 20, 2004; 110(3): 247 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Okuda, M. Yano, M. Doi, T. Oda, T. Tokuhisa, M. Kohno, S. Kobayashi, T. Yamamoto, T. Ohkusa, and M. Matsuzaki Valsartan Restores Sarcoplasmic Reticulum Function With No Appreciable Effect on Resting Cardiac Function in Pacing-Induced Heart Failure Circulation, February 24, 2004; 109(7): 911 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Scoote, P A Poole-Wilson, and A J Williams The therapeutic potential of new insights into myocardial excitation-contraction coupling Heart, April 1, 2003; 89(4): 371 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kohno, M. Yano, S. Kobayashi, M. Doi, T. Oda, T. Tokuhisa, S. Okuda, T. Ohkusa, M. Kohno, and M. Matsuzaki A new cardioprotective agent, JTV519, improves defective channel gating of ryanodine receptor in heart failure Am J Physiol Heart Circ Physiol, March 1, 2003; 284(3): H1035 - H1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yano, S. Kobayashi, M. Kohno, M. Doi, T. Tokuhisa, S. Okuda, M. Suetsugu, T. Hisaoka, M. Obayashi, T. Ohkusa, et al. FKBP12.6-Mediated Stabilization of Calcium-Release Channel (Ryanodine Receptor) as a Novel Therapeutic Strategy Against Heart Failure Circulation, January 28, 2003; 107(3): 477 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Scoote and A. J Williams The cardiac ryanodine receptor (calcium release channel): Emerging role in heart failure and arrhythmia pathogenesis Cardiovasc Res, December 1, 2002; 56(3): 359 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.E. Diaz, D.A. Eisner, and S.C. O'Neill Depressed Ryanodine Receptor Activity Increases Variability and Duration of the Systolic Ca2+ Transient in Rat Ventricular Myocytes Circ. Res., October 4, 2002; 91(7): 585 - 593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Doi, M. Yano, S. Kobayashi, M. Kohno, T. Tokuhisa, S. Okuda, M. Suetsugu, Y. Hisamatsu, T. Ohkusa, M. Kohno, et al. Propranolol Prevents the Development of Heart Failure by Restoring FKBP12.6-Mediated Stabilization of Ryanodine Receptor Circulation, March 19, 2002; 105(11): 1374 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Reiken, M. Gaburjakova, J. Gaburjakova, K.-l. He, A. Prieto, E. Becker, G.-h. Yi, J. Wang, D. Burkhoff, and A. R. Marks {beta}-Adrenergic Receptor Blockers Restore Cardiac Calcium Release Channel (Ryanodine Receptor) Structure and Function in Heart Failure Circulation, December 4, 2001; 104(23): 2843 - 2848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Hobai and B. O'Rourke Decreased Sarcoplasmic Reticulum Calcium Content Is Responsible for Defective Excitation-Contraction Coupling in Canine Heart Failure Circulation, March 20, 2001; 103(11): 1577 - 1584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hisaoka, M. Yano, T. Ohkusa, M. Suetsugu, K. Ono, M. Kohno, J. Yamada, S. Kobayashi, M. Kohno, and M. Matsuzaki Enhancement of Rho/Rho-kinase system in regulation of vascular smooth muscle contraction in tachycardia-induced heart failure Cardiovasc Res, February 1, 2001; 49(2): 319 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Eisner, H. S. Choi, M. E. Diaz, S. C. O'Neill, and A. W. Trafford Integrative Analysis of Calcium Cycling in Cardiac Muscle Circ. Res., December 8, 2000; 87(12): 1087 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M.N Terracciano Sarcoplasmic reticulum calcium release function and FK binding proteins in heart failure: another piece of a complex jigsaw Cardiovasc Res, November 1, 2000; 48(2): 191 - 193. [Full Text] [PDF]
|
|
|
|
|
|
K. Ono, M. Yano, T. Ohkusa, M. Kohno, T. Hisaoka, T. Tanigawa, S. Kobayashi, M. Kohno, and M. Matsuzaki Altered interaction of FKBP12.6 with ryanodine receptor as a cause of abnormal Ca2+ release in heart failure Cardiovasc Res, November 1, 2000; 48(2): 323 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yano, K. Ono, T. Ohkusa, M. Suetsugu, M. Kohno, T. Hisaoka, S. Kobayashi, Y. Hisamatsu, T. Yamamoto, M. Kohno, et al. Altered Stoichiometry of FKBP12.6 Versus Ryanodine Receptor as a Cause of Abnormal Ca2+ Leak Through Ryanodine Receptor in Heart Failure Circulation, October 24, 2000; 102(17): 2131 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.E. Diaz, A.W. Trafford, S.C. O'Neill, and D.A. Eisner Can changes of ryanodine receptor expression affect cardiac contractility? Cardiovasc Res, March 1, 2000; 45(4): 1068 - 1069. [Full Text] [PDF]
|
|
|
|
|
|
M. Yano and T. Yamamoto Abnormal sarcoplasmic reticulum Ca2+ release in heart failure Cardiovasc Res, March 1, 2000; 45(4): 1070 - 1071. [Full Text] [PDF]
|
|