Skip Navigation

Cardiovascular Research 1999 43(4):968-973; doi:10.1016/S0008-6363(99)00140-6
© 1999 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Kogaki, S.
Right arrow Articles by Okada, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kogaki, S.
Right arrow Articles by Okada, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1999, European Society of Cardiology

Selectin on activated platelets enhances neutrophil endothelial adherence in myocardial reperfusion injury

Shigetoyo Kogakia,*, Yoshiki Sawab, Tetsuya Sanoa, Tohru Matsushitaa, Toshihiro Ohatab, Shunji Kurotobia, Shinichiro J. Tojoc, Hikaru Matsudab and Shintaro Okadaa

aDepartment of Pediatrics, Faculty of Medicine, Osaka University 2-2, Yamada-oka Suita, Osaka 565-0871, Japan
bThe First Department of Surgery, Faculty of Medicine, Osaka University, Osaka, Japan
cSumitomo Pharmaceuticals Research Center, Osaka, Japan

* Corresponding author. Tel.: +81-6-879-3936; fax: +81-6-879-3939

Objectives: The glycoprotein P-selectin is an adhesion molecule that is rapidly expressed on the surface of platelets and endothelium during the inflammatory process. P-selectin on endothelium has been reported to play an important role in reperfusion injury. However, little is known regarding P-selectin on platelets in contributing to the pathophysiology of myocardial reperfusion injury. In this study, we hypothesized that P-selectin on platelets may enhance neutrophil endothelial adherence and this may play a role in neutrophil-mediated reperfusion injury. Methods: Endothelial cells, cardiomyocytes, platelets and neutrophils were isolated from adult rats. Endothelial cells and cardiomyocytes were cultivated in a co-culture system. After exposure to hypoxia and reoxygenation, neutrophil adherence and migration were examined. Results: After exposure to 6 h of hypoxia, endothelial cells co-incubated with platelets showed significantly greater neutrophil adherence (63.1±4.0%) and migration (78.2±6.7%) than endothelial cells alone (adhesion: 44.2±2.8%, migration: 57.9±4.9%). These increases were significantly inhibited (adhesion: 42.1±3.5%, migration: 65.5±3.8%) by an anti-P-selectin monoclonal antibody. Moreover, the superoxide-anion production was significantly elevated when activated platelets were added to neutrophils. This enhanced production was also inhibited by anti-P-selectin antibody. Conclusion: The presence of activated platelets enhanced neutrophil adhesion and migration process after hypoxia-reoxygenation. This process may occur following platelet-neutrophil interactions via P-selectin and subsequent neutrophil activation.

KEYWORDS Experimental; Heart; Pathophysiology; Cell communication; Leukocytes; Platelets; Reperfusion; Rat


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.