Skip Navigation

Cardiovascular Research 1999 43(4):892-900; doi:10.1016/S0008-6363(99)00040-1
© 1999 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bartfay, W. J.
Right arrow Articles by Liu, P. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bartfay, W. J.
Right arrow Articles by Liu, P. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1999, European Society of Cardiology

Cardiac function and cytotoxic aldehyde production in a murine model of chronic iron-overload

Wally J. Bartfaya, Fayez Dawooda, Wen H. Wena, Denis C. Lehotayb, David Houa, Emma Bartfayc, Xiaoping Luod, Peter H. Backxa and Peter P. Liua,*

aHeart and Stroke/Lewar Centre of Excellence in Cardiovascular Research, University of Toronto, Toronto, Ontario, Canada
bDepartment of Clinical Biochemistry, University of Toronto, Toronto, Ontario, Canada
cThe Institute For Health And Outcomes Research, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
dThe Hospital for Sick Children, Department of Clinical Biochemistry, Toronto, Ontario, Canada

* Corresponding author. Tel.: +1-416-340-3035; fax: +1-416-340-4753 peter.liu{at}utoronto.ca

Objectives: To determine the relationship between the total chronic dose of iron administered, ex-vivo cardiac function and the concentrations of cytotoxic aldehydes in heart tissue of a murine model. Methods: In the first experiment, 34 male B6D2F1 mice were randomized to receive intraperitoneal injections of 5, 10 or 20 mg of iron dextran for three weeks, or a placebo control. The mice were subsequently randomized to undergo ex-vivo assessment of cardiac function. In the second experiment, free radical generation, quantified by the presence of 20 separate cytotoxic aldehydes, was assessed in heart tissue of 40 mice that were randomized to receive chronic treatment with various concentrations of iron dextran (100 mg to 300 mg total chronic dose administered), placebo treatment with saline, or no treatment at all (baseline). Results: Iron-loaded groups displayed dose-dependent depressions of heart rate, systolic pressure, developed pressure, coronary pressure, –dP/dt and +dP/dt, and increases in diastolic pressure. Monotonic dose-dependent increases in total heart aldehydes were observed in the iron-treated groups (r=0.97, p<0.0001), whereas no significant differences were observed between baseline or time-placebo control groups. Conclusions: While no single mechanism is likely to account for the complex pathophysiology of iron-induced heart failure, our findings show that chronic iron-loading in a murine model results in dose-dependent alterations to cardiac function; and results in free radical mediated damage to the heart, as measured by excess concentrations of cytotoxic aldehyde-derived peroxidation products. This is the first description of the effects of excess iron on cardiac function assessed by an ex-vivo Langendorff technique in a murine model of chronic iron-overload.

KEYWORDS Iron-overload; Heart failure; Cardiac function; Free radicals; Aldehydes; Mice


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.