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Cardiovascular Research 1999 42(3):680-684; doi:10.1016/S0008-6363(99)00005-X
© 1999 by European Society of Cardiology
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Copyright © 1999, European Society of Cardiology

Ischemic preconditioning limits infarct size following regional ischemia–reperfusion in in situ mouse hearts

Diane L Millera and Donna M Van Winklea,b,*

aDepartment of Anesthesiology, Oregon Health Sciences University, Portland, OR, USA
bResearch and Anesthesiology Services, P-8-ANES VA Medical Center 3710 SW US Veterans Hospital Road, Portland, OR 97201 USA

vanwinkd{at}ohsu.edu

* Corresponding author. Tel.: +1-503-220-8262, ex 57404; fax: +1-503-721-7956

Objective: Ischemic preconditioning has been demonstrated in a wide variety of animals, from dogs to rats. Experimentally-induced murine myocardial ischemia–reperfusion has been described, but ischemic preconditioning has not been reported in mouse hearts. To test the hypothesis that mouse hearts exhibit preconditioning-induced protection, experiments were conducted in anesthetized open chest mice subjected to regional myocardial ischemia–reperfusion. Methods: Following barbiturate anesthesia the FVB and C57BL/6J mice underwent a tracheostomy and were mechanically ventilated. The heart was exposed via a left thoracotomy performed with the aid of a dissecting microscope. A 7-0 silk suture on a curved taper needle was passed under the proximal left anterior descending coronary artery to form a snare. Mice were then randomly assigned to receive either no preconditioning or preconditioning. All mice were subjected to 60 min regional myocardial ischemia followed by 2.5 h of reperfusion. Ischemic preconditioning (IP) was induced with two (FVB mice) or three (C57BL/6J mice) cycles of 5 min coronary occlusion and 5 min reperfusion. Control animals did not receive preconditioning ischemia. Area-at-risk was assessed with fluorescent particles. Infarct size was assessed with triphenyl tetrazolium chloride, and is expressed below as a percentage of the area-at-risk. Results: In FVB mice preconditioning reduced infarct size 49%, from 36.7±4.5% to 18.9±2.8% (P<0.05). In C57BL/6J mice preconditioning reduced infarct size by 66%, from 56.4±8.3% to 18.9±4.2% (P<0.05). Conclusion: From these data we conclude that the infarct limiting effect of ischemic preconditioning is demonstrable in murine hearts.

KEYWORDS Ischemic preconditioning; Myocardial infarct size; Mice


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