Skip Navigation

Cardiovascular Research 1999 42(3):680-684; doi:10.1016/S0008-6363(99)00005-X
© 1999 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Miller, D. L
Right arrow Articles by Van Winkle, D. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, D. L
Right arrow Articles by Van Winkle, D. M
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1999, European Society of Cardiology

Ischemic preconditioning limits infarct size following regional ischemia–reperfusion in in situ mouse hearts

Diane L Millera and Donna M Van Winklea,b,*

aDepartment of Anesthesiology, Oregon Health Sciences University, Portland, OR, USA
bResearch and Anesthesiology Services, P-8-ANES VA Medical Center 3710 SW US Veterans Hospital Road, Portland, OR 97201 USA

vanwinkd{at}ohsu.edu

* Corresponding author. Tel.: +1-503-220-8262, ex 57404; fax: +1-503-721-7956

Objective: Ischemic preconditioning has been demonstrated in a wide variety of animals, from dogs to rats. Experimentally-induced murine myocardial ischemia–reperfusion has been described, but ischemic preconditioning has not been reported in mouse hearts. To test the hypothesis that mouse hearts exhibit preconditioning-induced protection, experiments were conducted in anesthetized open chest mice subjected to regional myocardial ischemia–reperfusion. Methods: Following barbiturate anesthesia the FVB and C57BL/6J mice underwent a tracheostomy and were mechanically ventilated. The heart was exposed via a left thoracotomy performed with the aid of a dissecting microscope. A 7-0 silk suture on a curved taper needle was passed under the proximal left anterior descending coronary artery to form a snare. Mice were then randomly assigned to receive either no preconditioning or preconditioning. All mice were subjected to 60 min regional myocardial ischemia followed by 2.5 h of reperfusion. Ischemic preconditioning (IP) was induced with two (FVB mice) or three (C57BL/6J mice) cycles of 5 min coronary occlusion and 5 min reperfusion. Control animals did not receive preconditioning ischemia. Area-at-risk was assessed with fluorescent particles. Infarct size was assessed with triphenyl tetrazolium chloride, and is expressed below as a percentage of the area-at-risk. Results: In FVB mice preconditioning reduced infarct size 49%, from 36.7±4.5% to 18.9±2.8% (P<0.05). In C57BL/6J mice preconditioning reduced infarct size by 66%, from 56.4±8.3% to 18.9±4.2% (P<0.05). Conclusion: From these data we conclude that the infarct limiting effect of ischemic preconditioning is demonstrable in murine hearts.

KEYWORDS Ischemic preconditioning; Myocardial infarct size; Mice


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.