© 1999 by European Society of Cardiology
Copyright © 1999, European Society of Cardiology
Gene expression of proteins influencing the calcium homeostasis in patients with persistent and paroxysmal atrial fibrillation
aDepartment of Cardiology, Thoraxcenter, University Hospital Groningen, P.O. Box 30.001, 9700 RB Groningen, The Netherlands
bDepartment Clinical Pharmacology, Thoraxcenter, University Hospital Groningen, Groningen, The Netherlands
cDepartment of Thoracic Surgery, Thoraxcenter, University Hospital Groningen, Groningen, The Netherlands
i.c.van.gelder{at}thorax.azg.nl
* Corresponding author. Tel.: +31-50-3612355; fax: +31-50-3614391
Objective: Persistent atrial fibrillation (AF) results in an impairment of atrial function. In order to elucidate the mechanism behind this phenomenon, we investigated the gene expression of proteins influencing calcium handling. Methods: Right atrial appendages were obtained from eight patients with paroxysmal AF, ten with persistent AF (>8 months) and 18 matched controls in sinus rhythm. All controls underwent coronary artery bypass grafting, whereas most AF patients underwent Cox’s MAZE surgery (n=12). All patients had a normal left ventricular function. Total RNA was isolated and reversely transcribed into cDNA. In a semi-quantitative polymerase chain reaction the cDNA of interest and of glyceraldehyde-3-phosphate dehydrogenase were coamplified and separated by ethidium bromide-stained gel electrophoresis. Slot blot analysis was performed to study protein expression. Results: L-type calcium channel 
1 and sarcoplasmic reticulum Ca2+-ATPase mRNA (–57%, p=0.01 and –28%, p=0.04, respectively) and protein contents (–43%, p=0.02 and –28%, p=0.04, respectively) were reduced in patients with persistent AF compared to the controls. mRNA contents of phospholamban, ryanodine receptor type 2 and sodium/calcium exchanger were comparable. No changes were observed in patients with paroxysmal AF. Conclusions: Alterations in gene expression of proteins involved in the calcium homeostasis occur only in patients with long-term persistent AF. In the absence of underlying heart disease, the changes are rather secondary than primary to AF.
KEYWORDS Atrial fibrillation; Ca2+-handling proteins; Gene expression
Presented in part at the Congress of the NASPE (North American Society for Pacing and Electrophysiology), May 6–9 1998, San Diego, California.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Lenaerts, V. Bito, F. R. Heinzel, R. B. Driesen, P. Holemans, J. D'hooge, H. Heidbuchel, K. R. Sipido, and R. Willems Ultrastructural and Functional Remodeling of the Coupling Between Ca2+ Influx and Sarcoplasmic Reticulum Ca2+ Release in Right Atrial Myocytes From Experimental Persistent Atrial Fibrillation Circ. Res., October 23, 2009; 105(9): 876 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Qi, Y.-H. Yeh, D. Chartier, L. Xiao, Y. Tsuji, B. J.J.M. Brundel, I. Kodama, and S. Nattel The Calcium/Calmodulin/Kinase System and Arrhythmogenic Afterdepolarizations in Bradycardia-Related Acquired Long-QT Syndrome Circ Arrhythm Electrophysiol, June 1, 2009; 2(3): 295 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Michael, L. Xiao, X.-Y. Qi, D. Dobrev, and S. Nattel Remodelling of cardiac repolarization: how homeostatic responses can lead to arrhythmogenesis Cardiovasc Res, February 15, 2009; 81(3): 491 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mancarella, Y. Yue, E. Karnabi, Y. Qu, N. El-Sherif, and M. Boutjdir Impaired Ca2+ homeostasis is associated with atrial fibrillation in the {alpha}1D L-type Ca2+ channel KO mouse Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2017 - H2024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Y. Qi, Y.-H. Yeh, L. Xiao, B. Burstein, A. Maguy, D. Chartier, L. R. Villeneuve, B. J.J.M. Brundel, D. Dobrev, and S. Nattel Cellular Signaling Underlying Atrial Tachycardia Remodeling of L-type Calcium Current Circ. Res., October 10, 2008; 103(8): 845 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tsujimae, S. Murakami, and Y. Kurachi In silico study on the effects of IKur block kinetics on prolongation of human action potential after atrial fibrillation-induced electrical remodeling Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H793 - H800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K Nergardh, M. Rosenqvist, R. Nordlander, and M. Frick Maintenance of sinus rhythm with metoprolol CR initiated before cardioversion and repeated cardioversion of atrial fibrillation: a randomized double-blind placebo-controlled study Eur. Heart J., June 1, 2007; 28(11): 1351 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nattel, A. Maguy, S. Le Bouter, and Y.-H. Yeh Arrhythmogenic Ion-Channel Remodeling in the Heart: Heart Failure, Myocardial Infarction, and Atrial Fibrillation Physiol Rev, April 1, 2007; 87(2): 425 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shiroshita-Takeshita, B. J.J.M. Brundel, B. Burstein, T.-K. Leung, H. Mitamura, S. Ogawa, and S. Nattel Effects of simvastatin on the development of the atrial fibrillation substrate in dogs with congestive heart failure Cardiovasc Res, April 1, 2007; 74(1): 75 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Avila, I. M. Medina, E. Jimenez, G. Elizondo, and C. I. Aguilar Transforming growth factor-beta1 decreases cardiac muscle L-type Ca2+ current and charge movement by acting on the Cav1.2 mRNA Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H622 - H631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. El-Armouche, P. Boknik, T. Eschenhagen, L. Carrier, M. Knaut, U. Ravens, and D. Dobrev Molecular Determinants of Altered Ca2+ Handling in Human Chronic Atrial Fibrillation Circulation, August 15, 2006; 114(7): 670 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shiroshita-Takeshita, B. J.J.M. Brundel, J. Lavoie, and S. Nattel Prednisone prevents atrial fibrillation promotion by atrial tachycardia remodeling in dogs Cardiovasc Res, March 1, 2006; 69(4): 865 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pavlovic, A. Schaller, B. Steiner, P. Berdat, T. Carrel, J.-P. Pfammatter, R. A. Ammann, and S. Gallati Gender Modulates the Expression of Calcium-Regulating Proteins in Pediatric Atrial Myocardium Experimental Biology and Medicine, December 1, 2005; 230(11): 853 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Takagi, A Doi, N Shirai, K Hirata, Y Takemoto, K Takeuchi, and J Yoshikawa Acute improvement of atrial mechanical stunning after electrical cardioversion of persistent atrial fibrillation: comparison between biatrial and single atrial pacing Heart, January 1, 2005; 91(1): 58 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shiroshita-Takeshita, G. Schram, J. Lavoie, and S. Nattel Effect of Simvastatin and Antioxidant Vitamins on Atrial Fibrillation Promotion by Atrial-Tachycardia Remodeling in Dogs Circulation, October 19, 2004; 110(16): 2313 - 2319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hove-Madsen, A. Llach, A. Bayes-Genis, S. Roura, E. R. Font, A. Aris, and J. Cinca Atrial Fibrillation Is Associated With Increased Spontaneous Calcium Release From the Sarcoplasmic Reticulum in Human Atrial Myocytes Circulation, September 14, 2004; 110(11): 1358 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J.J.M Brundel, H. H Kampinga, and R. H Henning Calpain inhibition prevents pacing-induced cellular remodeling in a HL-1 myocyte model for atrial fibrillation Cardiovasc Res, June 1, 2004; 62(3): 521 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Van Noord, R. G. Tieleman, H. A. Bosker, T. Kingma, D. J. Van Veldhuisen, H. J.G.M Crijns, and I. C. Van Gelder Beta-blockers prevent subacute recurrences of persistent atrial fibrillation only in patients with hypertension Europace, January 1, 2004; 6(4): 343 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kinoshita, K. Sato, M. Hori, H. Ozaki, and H. Karaki Decrease in activity of smooth muscle L-type Ca2+ channels and its reversal by NF-{kappa}B inhibitors in Crohn's colitis model Am J Physiol Gastrointest Liver Physiol, August 8, 2003; 285(3): G483 - G493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Klein, F. Schroder, D. Vogler, A. Schaefer, A. Haverich, B. Schieffer, T. Korte, and H. Drexler Increased open probability of single cardiac L-type calcium channels in patients with chronic atrial fibrillation: Role of phosphatase 2A Cardiovasc Res, July 1, 2003; 59(1): 37 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shinagawa, A. Shiroshita-Takeshita, G. Schram, and S. Nattel Effects of Antiarrhythmic Drugs on Fibrillation in the Remodeled Atrium: Insights Into the Mechanism of the Superior Efficacy of Amiodarone Circulation, March 18, 2003; 107(10): 1440 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Bosch, C. R. Scherer, N. Rub, S. Wohrl, K. Steinmeyer, H. Haase, A. E. Busch, L. Seipel, and V. Kuhlkamp Molecular mechanisms of early electrical remodeling: transcriptional downregulation of ion channel subunits reduces ICa,L and Ito in rapid atrial pacing in rabbits J. Am. Coll. Cardiol., March 5, 2003; 41(5): 858 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E Deelman, G. Navis, E. de Boer, M. Wietses, D. de Zeeuw, and R. H Henning Role of proteinuria in the regulation of renal renin-angiotensin system components in unilateral proteinuric rats Journal of Renin-Angiotensin-Aldosterone System, March 1, 2003; 4(1): 38 - 42. [Abstract] [PDF]
|
|
|
|
|
|
R. F Bosch and S. Nattel Cellular electrophysiology of atrial fibrillation Cardiovasc Res, May 1, 2002; 54(2): 259 - 269. [Full Text] [PDF]
|
|
|
|
|
|
A. Shimizu and O. A. Centurion Electrophysiological properties of the human atrium in atrial fibrillation Cardiovasc Res, May 1, 2002; 54(2): 302 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J.J.M. Brundel, R. H. Henning, H. H. Kampinga, I. C. Van Gelder, and H. J.G.M. Crijns Molecular mechanisms of remodeling in human atrial fibrillation Cardiovasc Res, May 1, 2002; 54(2): 315 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel Therapeutic implications of atrial fibrillation mechanisms: can mechanistic insights be used to improve AF management? Cardiovasc Res, May 1, 2002; 54(2): 347 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yagi, J. Pu, P. Chandra, M. Hara, P. Danilo Jr., M. R Rosen, and P. A Boyden Density and function of inward currents in right atrial cells from chronically fibrillating canine atria Cardiovasc Res, May 1, 2002; 54(2): 405 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kneller, H. Sun, N. Leblanc, and S. Nattel Remodeling of Ca2+-handling by atrial tachycardia: evidence for a role in loss of rate-adaptation Cardiovasc Res, May 1, 2002; 54(2): 416 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shinagawa, H. Mitamura, S. Ogawa, and S. Nattel Effects of inhibiting Na+/H+-exchange or angiotensin converting enzyme on atrial tachycardia-induced remodeling Cardiovasc Res, May 1, 2002; 54(2): 438 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Smit-van Oosten, R. H Henning, and H. van Goor Strain differences in angiotensin-converting enzyme and angiotensin II type I receptor expression. Possible implications for experimental chronic renal transplant failure Journal of Renin-Angiotensin-Aldosterone System, March 1, 2002; 3(1): 46 - 53. [Abstract] [PDF]
|
|
|
|
|
|
V. L.J.L. Thijssen, J. Ausma, and M. Borgers Structural remodelling during chronic atrial fibrillation: act of programmed cell survival Cardiovasc Res, October 1, 2001; 52(1): 14 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vitadello, J. Ausma, M. Borgers, A. Gambino, D. C. Casarotto, and L. Gorza Increased Myocardial GRP94 Amounts During Sustained Atrial Fibrillation : A Protective Response? Circulation, May 1, 2001; 103(17): 2201 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. J. M. Brundel, I. C. Van Gelder, R. H. Henning, A. E. Tuinenburg, M. Wietses, J. G. Grandjean, A. A. M. Wilde, W. H. Van Gilst, and H. J. G. M. Crijns Alterations in potassium channel gene expression in atria of patients with persistent and paroxysmal atrial fibrillation: differential regulation of protein and mRNA levels for K+ channels J. Am. Coll. Cardiol., March 1, 2001; 37(3): 926 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun, D. Chartier, N. Leblanc, and S. Nattel Intracellular calcium changes and tachycardia-induced contractile dysfunction in canine atrial myocytes Cardiovasc Res, March 1, 2001; 49(4): 751 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fareh, A. Benardeau, and S. Nattel Differential efficacy of L- and T-type calcium channel blockers in preventing tachycardia-induced atrial remodeling in dogs Cardiovasc Res, March 1, 2001; 49(4): 762 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. J. M. Brundel, I. C. Van Gelder, R. H. Henning, R. G. Tieleman, A. E. Tuinenburg, M. Wietses, J. G. Grandjean, W. H. Van Gilst, and H. J. G. M. Crijns Ion Channel Remodeling Is Related to Intraoperative Atrial Effective Refractory Periods in Patients With Paroxysmal and Persistent Atrial Fibrillation Circulation, February 6, 2001; 103(5): 684 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Schotten, J. Ausma, C. Stellbrink, I. Sabatschus, M. Vogel, D. Frechen, F. Schoendube, P. Hanrath, and M. A. Allessie Cellular Mechanisms of Depressed Atrial Contractility in Patients With Chronic Atrial Fibrillation Circulation, February 6, 2001; 103(5): 691 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel and D. Li Ionic Remodeling in the Heart : Pathophysiological Significance and New Therapeutic Opportunities for Atrial Fibrillation Circ. Res., September 15, 2000; 87(6): 440 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M.W. van der Velden, J. Ausma, M. B. Rook, A. J.C.G.M. Hellemons, T. A.A.B. van Veen, M. A. Allessie, and H. J. Jongsma Gap junctional remodeling in relation to stabilization of atrial fibrillation in the goat Cardiovasc Res, June 1, 2000; 46(3): 476 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Van Wagoner, A. L. Pond, M. Lamorgese, S. S. Rossie, P. M. McCarthy, and J. M. Nerbonne Atrial L-Type Ca2+ Currents and Human Atrial Fibrillation Circ. Res., September 3, 1999; 85(5): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel Ionic Determinants of Atrial Fibrillation and Ca2+ Channel Abnormalities : Cause, Consequence, or Innocent Bystander? Circ. Res., September 3, 1999; 85(5): 473 - 476. [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel, D. M. Roden, and D. Escande A spotlight on electrophysiological remodeling and the molecular biology of ion channels Cardiovasc Res, May 1, 1999; 42(2): 267 - 269. [Full Text] [PDF]
|
|
|
|
|
|
C. Boixel, W. Gonzalez, L. Louedec, and S. N. Hatem Mechanisms of L-Type Ca2+ Current Downregulation in Rat Atrial Myocytes During Heart Failure Circ. Res., September 28, 2001; 89(7): 607 - 613. [Abstract] [Full Text] [PDF]
|
|