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Cardiovascular Research 1999 41(1):267-274; doi:10.1016/S0008-6363(98)00162-X
© 1999 by European Society of Cardiology
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Copyright © 1999, European Society of Cardiology

Identification of mRNA for 5-HT1 and 5-HT2 receptor subtypes in human coronary arteries

Tatsuro Ishida, Ken-ichi Hirata, Tsuyoshi Sakoda, Seinosuke Kawashima, Hozuka Akita and Mitsuhiro Yokoyama*

The First Department of Internal Medicine, Kobe University School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan

* Corresponding author. Tel.: +81-78-341-7451, ext. 5500; fax: +81-78-341-1390; e-mail: yokoyama@med.kobe-u.ac.jp

Objective: Although pharmacological studies have indicated that serotonin (5-HT)-evoked contraction of the human coronary artery is mediated by 5-HT1-like and 5-HT2 receptors, the gene expression of 5-HT receptors is still unclear. We examined mRNA expression of 5-HT1 and 5-HT2 receptor subtypes in human coronary arteries. Methods: Total RNA was extracted from human coronary arteries of 14 patients at autopsy by the guanidine method. Reverse transcription-polymerase chain reaction (RT–PCR) and ribonuclease protection assays were performed to identify 5-HT1 and 5-HT2 receptor mRNA expression in human coronary artery. Results: By RT–PCR, 5-HT1B, 5-HT2A and 5-HT2B mRNAs were detected in all of the 14 patients. 5-HT1A, 5-HT1D, and 5-HT1E mRNAs were detected in only some patients. However, neither 5-HT1F mRNA nor 5-HT2C mRNA was detected in any patient. By ribonuclease protection assay, 5-HT1B and 5-HT2A signals were detected in all patients examined, but neither 5-HT1A, 5-HT1D nor 5-HT2B signal was detected in any patient. Conclusions: Of 5-HT1/2 receptor subtypes, 5-HT1B and 5-HT2A receptor mRNAs were predominantly expressed in human coronary arteries. Our finding provides molecular evidence that the 5-HT1B receptor may be the 5-HT1-like receptor which mediates constriction of human coronary arteries.

KEYWORDS Human coronary artery; Serotonin; 5-HT1B receptor; 5-HT2A receptor; Ribonuclease protection assay


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