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Cardiovascular Research 1998 39(2):485-491; doi:10.1016/S0008-6363(98)00120-5
© 1998 by European Society of Cardiology
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Copyright © 1998, European Society of Cardiology

Peroxynitrite is not a major mediator of endothelial cell injury by activated neutrophils in vitro

Zhi Su, Hideyuki Ishida*, Naoto Fukuyama, Rumen Todorov, Chokoh Genka and Hiroe Nakazawa

Department of Physiology, Tokai University School of Medicine, Bohseidai, Kanagawa, 259-11, Japan

* Corresponding author. Tel.: +81 (463) 93 1121 (ext. 2531); Fax: +81 (463) 93 6684.

Objective: Human polymorphonuclear leukocytes (PMN) produce nitric oxide (NO), superoxide (O·–2) and peroxynitrite (ONOO–) upon stimulation. We investigated the role of ONOO– in PMN-induced injury to cultured bovine aortic endothelial cells (BAEC). Methods: BAEC were cocultured with phorbol 12-myristate 13-acetate (PMA)-activated human PMN (effector-to-target ratio, 10:1) and injury to BAEC was evaluated at intervals by 51Cr release assay. The levels of NO, O·–2, ONOO– and nitrotyrosine, a reaction product of ONOO–, were also measured, and the influence of NO synthase inhibitors, O·–2 and hydroxyl radical scavengers and other effectors was examined. Results: In BAEC cocultured with PMA-activated PMN, 51Cr release was significantly increased [14.6±2.2% at 2 h (p<0.05) and 42.6±2.7% at 4 h (p<0.01); control (nonactivated PMN), <4%]. Superoxide dismutase (100 U/ml) reduced 51Cr release to 4.6±2.2% at 2 h (p<0.05). N-Iminoethyl-L-ornithine (L-NIO, 0.1 mM) potentiated 51Cr release (30.6±3.8% at 2 h, p<0.01), and the potentiation was eliminated by anti-CD18 monoclonal antibody. The 51Cr release was completely prevented by dimethyl sulfoxide or by deferoxamine. Treatment of PMN with L-NIO inhibited NO generation and increased O·–2 production. The nitrotyrosine level did not increase in BAEC cocultured with PMA-activated PMN. Conclusion: NO-derived ONOO– is not a major cytotoxic mediator in BAEC injury by activated PMN. NO may have a cytoprotective effect by inhibiting PMN adherence to endothelial cells.

KEYWORDS Nitric oxide; Superoxide; Peroxynitrite; Tyrosine nitration; Neutrophils; Endothelial cells


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H.-Y. Sohn, F. Krotz, S. Zahler, T. Gloe, M. Keller, K. Theisen, T. M Schiele, V. Klauss, and U. Pohl
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