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Cardiovascular Research 1998 38(2):472-479; doi:10.1016/S0008-6363(98)00028-5
© 1998 by European Society of Cardiology
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Copyright © 1998, European Society of Cardiology

Myofilament calcium sensitivity is decreased in skinned cardiac fibres of endotoxin-treated rabbits

Benoît Taverniera,*, Delphine Garrigueb, Claire Boulleb, Benoît Valleta and Pascal Adnetb

aDépartement d'Anesthésie-Réanimation Chirurgicale 2, Centre Hospitalier Régional et Universitaire de Lille, 59037 Lille Cedex, France
bService d'Accueil des Urgences, Centre Hospitalier Régional et Universitaire de Lille, 59037 Lille Cedex, France

* Corresponding author. Département d'Anesthésie-Réanimation Chirurgicale 2, Hopital Huriez, Centre Hospitalier Régional et Universitaire, 59037 Lille Cedex, France. Tel.: +33 320 444508; fax: +33 320 444508.

Objective: The aim was to determine whether myofilament Ca2+ sensitivity is altered in skinned cardiac fibres from endotoxin-treated rabbits. Methods: Endotoxin was injected i.v. in conscious New-Zealand White rabbits at a dose of 0.5 mg/kg (groups I, II, and III) or 1 mg/kg (group IV). A fifth group was used as control. Hearts were excised 4 h (groups I and IV), 24 h (group II), or 5 days (Group III) after injection. Skinned fibres were obtained with chemical (EGTA+Brij 58) treatment of bundles isolated from papillary muscles. Results: The maximal Ca2+-activated force (F0) of skinned cardiac fibres was not different between groups. However, [Ca2+] required to evoke 50% of F0 (Ca50) was higher in the fibres from group I than in controls (1.78±0.05 vs. 1.53±0.03 µM; P<0.05). This effect was dose-dependent (group IV: Ca50=2.08±0.12 µM; P<0.05 vs. group I), larger after 24 h (group II: Ca50=2.12±0.05 µM; P<0.05 vs. group I). By 5 days, myofilament Ca2+ sensitivity had returned to normal (group III: Ca50=1.54±0.05 µM). Conclusion: Myofilament Ca2+ sensitivity is decreased in skinned fibres taken from rabbit myocardium after i.v. endotoxin injection. This effect is dose- and time-dependent, and reversible with time.

KEYWORDS Endotoxin; Cardiac function; Contractile apparatus; Calcium ion; Rabbit


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