Skip Navigation

Cardiovascular Research 1998 38(2):301-315; doi:10.1016/S0008-6363(98)00026-1
© 1998 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Doevendans, P. A.
Right arrow Articles by van Bilsen, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doevendans, P. A.
Right arrow Articles by van Bilsen, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1998, European Society of Cardiology

Hunting down nucleic acid binding factors in the cardiovascular system

Pieter A. Doevendansa,*, Pilar Ruiz-Lozanoc and Marc van Bilsenb

aDept. of Cardiology, Cardiovascular Research Institute Maastricht, Maastricht, Netherlands
bDept. of Physiology, Cardiovascular Research Institute Maastricht, Maastricht, Netherlands
cDept. of Medicine, Center for Molecular Genetics, UCSD, La Jolla, CA, USA

* Corresponding author. Tel.: +31 (43) 387 5095; Fax: +31 (43) 387 5104; E-mail: pieter.doevendans@cardio.azm.nl

Transcription regulation of genes active in the cardiovascular system is a complex process, involving DNA and RNA binding proteins. Nucleic acid binding proteins bind to the regulatory DNA and interact with other proteins, including RNA polymerase to initiate and control the level of transcription. The RNA binding proteins have a function in spliceosome formation and in stabilising mRNA. In this review the currently available molecular approaches to analyse regulatory DNA in relation to DNA binding proteins are discussed. Similar techniques that have been developed for RNA binding protein studies are included. In addition to an explanation of the various methods, examples are provided from DNA-protein interactions on genes active in the cardiovascular system, together with strategies for identification and characterisation of new nucleic acid binding proteins active in cardiac or vascular cell types.

KEYWORDS Transcription factors; DNA-protein binding; RNA binding proteins; Transient transfection; Transgenesis; Mouse; Transcription initiation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.