© 1998 by European Society of Cardiology
Copyright © 1998, European Society of Cardiology
Activation of mitogen-activated protein kinases and activator protein-1 in myocardial infarction in rats
aFirst Department of Internal Medicine, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, Osaka 545-0051, Japan
bDepartment of Pharmacology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, Osaka 545-0051, Japan
* Corresponding author. Tel. (+81-6) 645 2106; Fax (+81-6) 645 2107.
Objective: The purpose of this study was to examine the activation of mitogen-activated protein kinases (MAPK) plus activator protein-1 (AP-1) and nuclear factor-
B (NF-B) DNA binding activities, all of which seem to be important in a signal transduction cascade upstream of the increased level of mRNA expression observed after myocardial infarction. Methods: Myocardial infarction was produced in Wistar rats. The activities of MAPKs in the ischemic region were measured using an in-gel kinase method or an in vitro kinase method. AP-1 and NF-B binding was determined using an electrophoretic mobility shift assay. Levels of transforming growth factor β1(TGF-β1) and collagen I and III mRNAs were analyzed by Northern blot hybridization. Results: p42 Extracellular signal-regulated kinase (ERK), p44ERK and p38MAPK activities increased 5.2-fold, 4.3-fold and 1.9-fold (P<0.01), respectively, at 5 min after coronary artery ligation but returned to normal levels by 30 min. p55 c-Jun NH2-terminal kinase (JNK) and p46JNK activities increased 4.0-fold and 3.2-fold (P<0.01), respectively, at 15 min and returned to normal levels by 24 h after ligation. AP-1 DNA and NF-B binding activities increased 8.7-fold and 7.1-fold (P<0.01), respectively, at 3 days but returned to normal levels by 7 days after ligation. Interestingly, analyses of the levels of TGF-β1, collagen I and III mRNAs revealed increases of 6.3-fold, 15.2-fold and 12.0-fold (P<0.01), respectively, at 1 week after myocardial infarction. Conclusions: Myocardial ischemia increased MAPK activities, which were followed by enhancement of AP-1 and NF-B DNA binding activity in areas of myocardial infarction in rats. These signal transduction mechanisms may contribute to the myocardial ischemia and injury associated with myocardial infarction by causing an increased expression of TGF-β1 mRNA, collagen I and III in the area.
KEYWORDS Myocardial infarction; Mitogen-activated protein kinase; Extracellular signal-regulated kinase; c-Jun NH2-terminal kinase; p38MAPK; Activator protein-1; Nuclear factor-B; Transforming growth factor β1; Collagen I; Collagen III; Ternary complex factor/Elk-1; Serum response factor
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