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Cardiovascular Research 1998 37(3):606-617; doi:10.1016/S0008-6363(97)00238-1
© 1998 by European Society of Cardiology
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Copyright © 1998, European Society of Cardiology

Expression of calcium regulatory proteins in short-term hibernation and stunning in the in situ porcine heart1

Hartmut Lüssa,*, Peter Bokniéka, Gerd Heuschb, Frank Ulrich Müllera, Joachim Neumanna, Wilhelm Schmitza and Rainer Schulzb

aInstitut für Pharmakologie und Toxikologie, Westfälische Wilhelms-Universität Münster, Domagkstr. 12, D-48129 Münster, Germany
bAbteilung für Pathophysiologie, Zentrum für Innere Medizin des Universitätsklinikums Essen, D-45122 Essen, Germany

* Corresponding author. Tel. (+49-251) 835 5510; Fax (+49-251) 835 5501.

Background: Myocardial hibernation and stunning are characterised by a reversible contractile dysfunction during and after ischaemia, respectively. Calcium homeostasis might be disturbed in hibernation and stunning due to altered expression of cardiac proteins involved in calcium handling. Methods: In enflurane-anaesthetised swine the coronary blood flow through the left anterior descending coronary artery was decreased to reduce regional contractile function (microsonometry) by ~50%. In transmural biopsies obtained during ischaemia and reperfusion creatine phosphate as well as the expression of sarcoplasmic reticulum calcium ATPase (SERCA), phospholamban (PLB), calsequestrin (CSQ), and troponin inhibitor (TnI) were determined. Results: During ischaemia creatine phosphate, after an initial reduction, recovered back to control values, and necrosis was absent (hibernation). After 90 min of ischaemia the myocardium was reperfused for 120 min but regional contractile function continued to be depressed (stunning). PLB, SERCA, CSQ, and TnI proteins were unchanged during ischaemia as well as reperfusion. Likewise, levels of PLB and SERCA mRNAs were unchanged. Conclusion: It is concluded that other mechanisms than altered expression of these regulating proteins underlie the contractile dysfunction observed during acute ischaemia, short-term hibernation and stunning.

KEYWORDS Calsequestrin; Short-term hibernation; Ischaemia; Phospholamban; Reperfusion; Sarcoplasmic reticulum calcium ATPase; Swine; Troponin inhibitor

1 Dedicated to Professor Dr. Hasso Scholz, Hamburg, on the occasion of his 60th birthday.


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