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Cardiovascular Research 1998 37(1):160-170; doi:10.1016/S0008-6363(97)00220-4
© 1998 by European Society of Cardiology
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Copyright © 1998, European Society of Cardiology

Metabolic alterations in the chronically denervated dog heart

Ger J van der Vussea,*, Marie-Louise Dubelaara, Will A Coumansa, Anne-Marie L Seymourb, Sinead B Clarkec, Arend Bonend, Angela J Drake-Hollandc and Mark I.M Noblec

aDepartment of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, P.O. Box 616, 6200 MD Maastricht, Netherlands
bDepartment of Thoracic Surgery, National Heart Lung Institute at Harefield Hospital, Harefield, Middlesex, UK
cAcademic Unit of Cardiopulmonary Medicine, Charing Cross and Westminster Medical School, University of London, London, UK
dDepartment of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada

* Corresponding author. Tel. +31-43-3881086; Fax +31-43-3671028; E-mail: vandervusse@fys.unimaas.nl

Objectives: Previous studies have shown that chronic cardiac denervation impairs myocardial glucose oxidation. To investigate this further we tested whether the tissue content of glucose transporters, activity of glycolytic enzymes or metabolic capacity of pyruvate dehydrogenase were altered. Moreover, we investigated whether the decline in glucose utilization was associated with an upregulation of proteins and enzymes involved in fatty acid handling. Chronic cardiac denervation results also in decreased left ventricular efficiency. We explored whether alterations in mitochondrial properties could be held responsible for this phenomenon. Methods: Twelve adult dogs were included in the study. In 6 of them chronic cardiac denervation was accomplished by surgical ablation of the extrinsic nerve fibers. The other 6 dogs were sham-operated. Biopsies were obtained from the left ventricle after 4–5 weeks of denervation. The content or enzymatic activity of proteins involved in fatty acid and glucose handling was assessed. Features of glutamate oxidation were measured in freshly isolated mitochondria. Results: The content or activity of a set of fatty acid handling proteins did not change during chronic cardiac denervation. In contrast GLUT1 content significantly increased in the chronically denervated left ventricle, while the active form of pyruvate dehydrogenase declined (p<0.05). Glutamate oxidation characteristics in freshly isolated mitochondria were not affected by chronic denervation. Conclusion: The impairment of glucose oxidation in the chronically denervated myocardium is most likely caused by a decline of pyruvate dehydrogenase in its active form. It is unlikely that the decrease in work efficiency is caused by alterations in mitochondrial properties.

KEYWORDS Chronic denervation; Dog; Pyruvate dehydrogenase; Mitochondria; GLUT1; GLUT4; Carnitine acyl transferase


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