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Cardiovascular Research 1997 35(3):553-559; doi:10.1016/S0008-6363(97)00161-2
© 1997 by European Society of Cardiology
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Copyright © 1997, European Society of Cardiology

Expression and function of a recombinant endothelial nitric oxide synthase gene in porcine coronary arteries

David G Cablea, Timothy O'Brienb, Iftikhar J Kulloc, Robert S Schwartzc, Hartzell V Schaffd and Vincent J Pompilie,*

aCardiac Surgical Research Center, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
bDivision of Endocrinology, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
cDivision of Cardiovascular Disease, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
dSection of Cardiovascular Surgery, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
eKrannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

* Corresponding author. Krannert Institute of Cardiology, Indiana University Medical Center, 1111 West 10th Street, Indianapolis, IN 46202, USA. Tel.: +1 (317) 630-2483; fax: +1 (317) 630-7449; e-mail: pompili@kimail.dmed.iupui.edu

Objectives: Direct gene transfer of exogenous nitric oxide synthase, with the subsequent increase in nitric oxide production, could represent a potential therapeutic strategy in the treatment of vascular proliferative disorders. The goal of the present study was to determine if porcine coronary arteries could be transduced with an adenoviral vector encoding endothelial nitric oxide synthase (Ad.CMVeNOS) resulting in functional expression. Methods and Results: Segments of porcine right coronary artery were exposed for 1 h at 37°C to either replication-deficient adenovirus encoding bovine endothelial nitric oxide synthase (Ad.CMVeNOS, 5x109 pfu/ml) or control adenovirus encoding Escherichia coli β-galactosidase (Ad.CMVLacZ, 5x109 pfu/ml). Immunohistochemistry with a monoclonal antibody specific for nitric oxide synthase (NOS) demonstrated recombinant gene expression in both the endothelial and adventitial layers of Ad.CMVeNOS transduced coronaries with only endogenous NOS confirmed in the endothelium of Ad.CMVLacZ arteries. Coronary arteries transduced with Ad.CMVeNOS yielded 517±110 (mean±S.E.M.) nM/ng nitrite while vessels transduced with Ad.CMVLacZ yielded 126±84 nM/ng (P<0.05, n = 6). Isometric tension recording, following prostaglandin F2{alpha} constriction, documented a reduced tension in Ad.CMVeNOS transduced coronaries, compared to matched Ad.CMVLacZ coronaries (6.10±1.08 g vs. 8.45±1.19 g, respectively, P = 0.05, n = 8). This tension differential was eliminated with prior incubation in NG-monomethyl-L-arginine (L-NMMA, 10–4 M). The EC50 for calcium ionophore relaxation of Ad.CMVeNOS coronary arteries was reduced compared to Ad.CMVLacZ (–7.90±0.03 logM vs. –7.26±0.11 logM, respectively, P<0.05, n = 8). Conclusions: These studies demonstrate successful transfer of endothelial nitric oxide synthase into porcine coronary arteries as verified by histochemical localization of recombinant protein with an increase of nitric oxide release as demonstrated by enhanced nitrite production and an alteration in vasomotor function.

KEYWORDS Nitric oxide synthase; Adenoviral vector; Gene therapy; Porcine coronary artery; Vasomotor function


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