Skip Navigation

Cardiovascular Research 1997 35(1):99-105; doi:10.1016/S0008-6363(97)00089-8
© 1997 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Regitz-Zagrosek, V.
Right arrow Articles by Fleck, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Regitz-Zagrosek, V.
Right arrow Articles by Fleck, E.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1997, European Society of Cardiology

Angiotensin receptor type 1 mRNA in human right ventricular endomyocardial biopsies: downregulation in heart failure

Vera Regitz-Zagroseka,*, Jens Fielitza, R Dreyssea, Alfred G Hildebrandtb and Eckart Flecka

aInnere Medizin/Kardiologie, Virchow-Klinikum, Humboldt Universität and Deutsches Herzzentrum, Augustenburger Platz 1, 13353 Berlin, Germany
bBundesinstitut für Arzneimittel, Berlin, Germany

* Corresponding author. Tel.: +49 (30) 4593 1000; fax: +49 (30) 4593 2500; e-mail: regitz@ukrv.de

Objective: Atrial angiotensin II receptors type 1 (AT1) are downregulated in end-stage human heart failure at mRNA and protein level. The present study investigated whether AT1 ventricular mRNA content was reduced in myocardial biopsies from heart failure patients. Methods: AT1 mRNA was quantitated in right ventricular endomyocardial biopsies from 16 patients with decreased left ventricular function (LVEF 36±3%) due to dilated cardiomyopathy (DCM) and in biopsies from 12 patients with suspected myocardial disease but normal cardiac function (LVEF 62±2%). Two biopsies per patient were pooled, RNA was extracted and reverse-transcribed after addition of an AT1 cRNA standard. AT1 standard and wild-type RNA were amplified with the same primers in the same PCR tube. The PCR products were hybridized to a microtiter plate and detected and quantitated by an ELISA system. Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA was determined in the same samples as AT1 mRNA. Results: In the biopsies from 16 patients with heart failure, a 68% decrease in AT1 mRNA content was found in comparison with 12 controls (heart failure 94±15 AT1 mRNA copies/ng RNA; controls 297±45; P<0.001). Relating AT1 mRNA content to GAPDH mRNA confirmed the specific decrease in AT1 mRNA (AT1/GAPDH: heart failure 1.3±0.15; controls 3.4±0.5; P<0.002). The best correlation between AT1 mRNA content and clinical parameters was found for right ventricular ejection fraction (r = 0.59, P<0.01). Conclusions: The quantitative RT-PCR procedure indicated a loss of ventricular AT1 mRNA in human heart failure which corresponds to the loss of AT1 protein described previously. It may underlie the decrease in AT1 protein expression in human heart failure.

KEYWORDS Human; Angiotensin receptor; mRNA; RT-PCR


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
J. W. Adams, J. Wang, J. R. Davis, C. Liaw, I. Gaidarov, J. Gatlin, N. D. Dalton, Y. Gu, J. Ross Jr., D. Behan, et al.
Myocardial expression, signaling, and function of GPR22: a protective role for an orphan G protein-coupled receptor
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H509 - H521.
[Abstract] [Full Text] [PDF]


Home page
Eur J Heart FailHome page
M. Maczewski, M. Borys, P. Kacprzak, T. Gdowski, and D. Wojciechowski
Angiotensin II AT1 receptor density on blood platelets predicts early left ventricular remodelling in non-reperfused acute myocardial infarction in humans
Eur J Heart Fail, March 1, 2006; 8(2): 173 - 178.
[Abstract] [Full Text] [PDF]


Home page
Journal of Renin-Angiotensin-Aldosterone SystemHome page
J. L Mehta and Dayuan Li
Facilitative interaction between angiotensin II and oxidised LDL in cultured human coronary artery endothelial cells
Journal of Renin-Angiotensin-Aldosterone System, March 1, 2001; 2(1_suppl): S70 - S76.
[Abstract] [PDF]



Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.