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Cardiovascular Research 1995 30(5):799-806; doi:10.1016/S0008-6363(95)00128-X
© 1995 by European Society of Cardiology
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Copyright © 1995, European Society of Cardiology

Effect of sulfhydryl oxidation on ionic and gating currents associated with L-type calcium channels in isolated guinea-pig ventricular myocytes

Alain Lacampagnea, Anne Duittoza, Pura Bolañosb, Nicolas Peineaua and Jorge A. Argibay*

aPhysiologie des Cellules Cardiaques et Vasculaires C.N.R.S. EP 21, Faculté des Sciences, Parc de Grandmont, 37200 Tours, France
bCentro de Biofísica y Bioquímica, I.V.I.C., Caracas, Venezuela

* Corresponding author. Tel. (+ 47) 36.70.09; Fax (+ 47) 36.71.12.

Objective: L-type calcium currents (Ica) and gating currents modification by extracellular application of the selective free sulfhydryl oxidant p-hydroxy-mercuric-phenylsulphonic acid (PHMPS) were studied. Methods: Both currents were obtained with the whole cell patch clamp technique in guinea-pig ventricular cardiocytes. Results: The main finding was a reduction of Ica clearly differentiable from a "run down" process. This effect was protected, stopped and in some cases partially reversed by dithiothreitol, a protective reagent for -SH groups. We also found a decrease of the gating currents associated with L-type calcium channels. The calcium modulation and cAMP phosphorylation systems of Ica are unaffected by PHMPS. With barium as charge carrier the current-voltage curves of barium currents were shifted by 10 mV to the positive direction by PHMPS. The same effect was obtained with calcium currents using BAPTA as a fast calcium buffer. Conclusion: The results indicate that oxidation of -SH groups carried by the channel protein induces dysfunction of the calcium entry to cardiac cells by altering the gating process. A participation of thiol functions on the gating of the calcium channel is proposed.

KEYWORDS Calcium channel, L-type; Guinea pig, ventricular myocytes; Sulfhydryl groups


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