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Cardiovascular Research 1995 29(6):834-840; doi:10.1016/S0008-6363(96)88620-2
© 1995 by European Society of Cardiology
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Copyright © 1995, European Society of Cardiology

Quantification and localisation of angiotensin II receptors and angiotensin converting enzyme in the developing rat heart

Rachel A Hunta, Gladys M Ciuffob, Juan M Saavedrab and Diane C Tucker*,a

aDepartment of Psychology, University of Alabama at Birmingham, 218 Campbell Hall, Birmingham, AL 35294, USA
bSection on Pharmacology, Laboratory of Clinical Sciences, National Institute of Mental Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

* Correspondence to Dr Tucker (Building 10, Room 2D-45).

Objective: The aim was to determine the quantification and localisation of angiotensin II receptors and angiotensin converting enzyme (ACE) in the developing rat heart. Methods: Quantitative autoradiography was used to determine the density of [125I]Sar1-angiotensin II binding to angiotensin II receptors and the density of [125I]351A binding to ACE. Results: Angiotensin II receptors were first detected in the myocardium on embryonic day 14, and reached a maximum density within the first postnatal week. During the perinatal period, the density of angiotensin II receptors was twofold higher in atrial than in ventricular myocardium [27(SEM 7) {nu} 11(1) fmol·mg–1 protein]. By adulthood, however, the density of angiotensin II receptors did not differ between atria and ventricles. A high density of angiotensin II receptors was first detected in the cardiac vasculature on embryonic day 19, with binding density decreasing during development. During the embryonic and early postnatal periods, most of the binding in the cardiac vasculature was to AT2 receptors. ACE was first detected in the myocardium on the day of birth, with the density of binding to ACE increasing during development. On postnatal day 56, the density of binding to ACE was fourfold higher in atrial than in ventricular myocardium [99(22) {nu} 22(4) fmol·mg–1 protein]. A moderate density of binding to ACE was first detected in the cardiac vasculature and heart valves on embryonic day 19, with binding density increasing during development. By adulthood, the density of binding to ACE was 10- to 25-fold higher in the cardiac vasculature and heart valves than in the myocardium. Conclusions: The density of angiotensin II receptors and the density of binding to ACE are developmentally regulated in the heart. Both AT1 and AT2 receptors were present in the rat heart by late gestation and could, therefore, mediate the effects of angiotensin II on early cardiac growth and development.

KEYWORDS angiotensin II receptors; angiotensin converting enzyme; quantitative autoradiography; cardiac development

{star} This work was supported in part by U.S. Public Health Service grants HL 39048 and HL 42258, and an American Heart Association grant-in-aid (DCT).


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