© 1995 by European Society of Cardiology
Copyright © 1995, European Society of Cardiology
Neutrophil activation and adhesion molecule expression in a canine model of open heart surgery with cardiopulmonary bypass
aSperos P Martel Section of Leukocyte Biology and Inflammation Research Baylor College of Medicine, Houston, Texas, USA
bSection of Cardiovascular Sciences Baylor College of Medicine, Houston, Texas, USA
* Correspondence to Dr Dreyer, at Pediatric Cardiology, Texas Children's Hospital, 6621 Fannin, Houston, TX 77030, USA.
Objective: The aim was to determine whether, in a canine model, changes in surface expression of the neutrophil adhesion molecules CD11b/CD18 and L-selectin during and after open heart surgery with cardiopulmonary bypass can be used to identify subjects at risk for postoperative pulmonary dysfunction. Methods: Adult mixed breed dogs underwent cardiopulmonary bypass and were compared to "sham bypass" controls. Flow cytometry was performed on blood from the two groups of dogs and changes in CD11b/CD18 adhesion molecules and L-selectin were investigated. Results: Flow cytometry on blood from bypass dogs showed increased CD18 expression during and after cardiopulmonary bypass and a reciprocal decrease in L-selectin expression. Sham animals showed no significant change. In the bypass animals, changes in adhesion molecule expression were not evenly distributed across the population of circulating neutrophils; however, they were indicative of a percentage of activated cells. There was a significant negative linear relationship between the percentage of activated cells and arterial oxygenation 3 h after bypass (r = –0.80, P < 0.001). From this analysis, 11 animals were identified as "high" responders and seven as "low" responders, with different patterns of cellular activation and oxygenation during and after bypass. High responders had an average of 40(SEM 5)% activated cells during bypass with a persistently raised percentage of activated cells [38(3)%] 3 h later, whereas low responders had only 22(6)% activated cells during bypass and 11(2)% activated cells 3 h after bypass. High responder animals had a marked and continued deterioration in Po2 after bypass [to 25(6)% of baseline 3 h after bypass] whereas low responder animals showed recovery of oxygenation after the first hour postbypass and improved to 80(8)% of baseline at 3 h. Conclusions: Changes in adhesion molecule expression serve as a marker of neutrophil activation during cardiopulmonary bypass. The percentage of activated neutrophils in the circulation within 3 h after cardiopulmonary bypass may be predictive of an ongoing inflammatory process that is linked to pulmonary dysfunction.
KEYWORDS neutrophils; complement; inflammation; CD11b/CD18; L-selectin; cardiopulmonary bypass
This work was supported in part by grants HL-47163 and HL-42550 from the National Institutes of Health. Dr Dreyer's work was done during the tenure of a Clinician-Scientist Award from the American Heart Association and Boehringer Ingelheim Pharmaceuticals, Inc.
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