© 1993 by European Society of Cardiology
Copyright © 1993, European Society of Cardiology
Cholesterol induced lipid accumulation in myocardial cells of rats
University of Bergen, Årstadveien 19, N-5009 Bergen, Norway—Department of Anatomy: S Hexeberg, S Rotevatn; Laboratory of Clinical Biochemistry, Haukeland Sykehus: N Willumsen, R K Berge; Department of Surgery, Haukeland Sykehus: E Hexeberg.
Correspondence to Dr Sofie Hexeberg.
Objective: The aim was to investigate whether cholesterol feeding of rats for only 10 d would result in lipid accumulation in myocardial cells and a change in the peroxisomal β oxidation in the heart compared to the hearts of rats on standard feeds. Methods: Eight rats received a cholesterol diet (2%) and eight rats received a standard diet for 10 d. Lipids were measured in serum, liver, and heart. Palmitoyl-CoA hydrolase and fatty acyl-CoA oxidase were determined in total homogenate of hearts. The fractional volume of lipid droplets in myocardial cells was calculated from micrographs at a magnification of x9600 obtained by electron microscopy. Results: The fractional volume of lipid droplets in the cardiomyocytes increased from 0.109(SEM 0.019)% to 0.259(0.037)%, (p<0.003), as a result of cholesterol feeding. Cholesterol and triglycerides in the heart measured by biochemical methods increased by 13% and 24%, respectively. There was no difference in palmitoyl-CoA hydrolase or fatty acyl-CoA oxidase in the hearts of the cholesterol fed group compared to the control group, suggesting an unaltered peroxisomal β oxidation of fatty acids in the myocardium. The serum triglycerides and serum phospholipids were reduced in the cholesterol fed rats (p<0.05 and p<0.001, respectively). Rats fed cholesterol diet showed a reduced ratio of HDL cholesterol to total cholesterol in serum, together with an increased liver weight and relative liver weight compared to the control rats. The addition of cholesterol to the diet increased liver cholesterol to 21.0(1.6) µmol·g–1 wet weight compared to 5.87(0.93) µmol·g–1 in the controls on standard diet (p<0.0001). Cholesterol feeding had no effect on the hepatic level of phospholipids, but the liver triglycerides tended to increase from 23.3(9.5) µmol·g–1 wet weight to 53.3(6.3) µmol·g–1. Conclusions: Lipid accumulation in the myocardial cells was shown by morphometry after 10 d of cholesterol feeding, without any visible damage to the tissue.
Cardiovascular Research 1993;27:442-446
KEYWORDS cardiomyocytes; cholesterol diet; electron microscopy; lipid accumulation; morphometry; rat
The work has been supported by the Norwegian Council on Cardiovascular Diseases (research fellowship for SH) and by the Norwegian Cancer Society (research fellowship for NW). The authors are grateful to Edel-Karin Frotjold, Elisabeth Sandvik, Svein Kryger, Terje Bjørndal, and Aase Heltveit for technical assistance.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Ferdinandy, R. Schulz, and G. F. Baxter Interaction of Cardiovascular Risk Factors with Myocardial Ischemia/Reperfusion Injury, Preconditioning, and Postconditioning Pharmacol. Rev., December 1, 2007; 59(4): 418 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Csont, E. Bereczki, P. Bencsik, G. Fodor, A. Gorbe, A. Zvara, C. Csonka, L. G. Puskas, M. Santha, and P. Ferdinandy Hypercholesterolemia increases myocardial oxidative and nitrosative stress thereby leading to cardiac dysfunction in apoB-100 transgenic mice Cardiovasc Res, October 1, 2007; 76(1): 100 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Giricz, M. M. Lalu, C. Csonka, P. Bencsik, R. Schulz, and P. Ferdinandy Hyperlipidemia Attenuates the Infarct Size-Limiting Effect of Ischemic Preconditioning: Role of Matrix Metalloproteinase-2 Inhibition J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 154 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ungi, T. Ungi, Z. Ruzsa, E. Nagy, Z. Zimmermann, T. Csont, and P. Ferdinandy Hypercholesterolemia Attenuates the Anti-ischemic Effect of Preconditioning During Coronary Angioplasty Chest, September 1, 2005; 128(3): 1623 - 1628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Onody, C. Csonka, Z. Giricz, and P. Ferdinandy Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts Cardiovasc Res, June 1, 2003; 58(3): 663 - 670. [Abstract] [Full Text] [PDF]
|
|