© 1993 by European Society of Cardiology
Copyright © 2003, European Society of Cardiology
The relative contributions of different intracellular and sarcolemmal systems to relaxation in rat ventricular myocytes
Department of Veterinary Preclinical Sciences, University of Liverpool, PO Box 147, Liverpool L69 3BX, United Kingdom: N Negretti, S C O'Neill, D A Eisner.
Correspondence to Professor Eisner.
Objective: The aim was to estimate the relative contributions of the various intracellular and sarcolemmal systems to the relaxation of the systolic calcium transient. Methods: The experiments were performed on isolated rat ventricular myocytes. The cells were loaded with the fluorescent indicator indo-1 in order to measure [Ca2+]i. Results: The application of caffeine to release calcium from the sarcoplasmic reticulum produced a rise of [Ca2]i which decayed about 7-8 times more slowly than the electrically stimulated calcium transient. This suggests that the sarcoplasmic reticulum accounts for about 87% of the calcium removal. The rate of decay of the caffeine response was decreased to about 33% of the control by inhibiting the Na-Ca exchange with Ni2+. In the presence of Ni2+ the rate could be inhibited further by inhibiting either the sarcolemmal Ca-ATPase (by increasing extracellular calcium concentration, [Ca2+]o) or the mitochondria (with FCCP and oligomycin). The relative contributions of the various processes were estimated to be: sarcoplasmic reticulum 87%, mitochondria 1.7%, Na-Ca 8.7%, sarcolemmal Ca-ATPase 2.6%. Conclusions: These experiments show that the Na-Ca exchange accounts for 67% of the calcium removal not mediated by the sarcoplasmic reticulum. This is a smaller fraction than in rabbit cardiac cells and highlights the importance of the Ca-ATPase in the rat heart.
Cardiovascular Research 1993;27;1826-1830
KEYWORDS calcium; contraction; Na-Ca
The work described in this paper was supported by grants from the British Heart Foundation and the Wellcome Trust. NN was supported by the Universidad Central de Venezuela.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Nakajima-Takenaka, G.-X. Zhang, K. Obata, K. Tohne, H. Matsuyoshi, Y. Nagai, A. Nishiyama, and M. Takaki Left ventricular function of isoproterenol-induced hypertrophied rat hearts perfused with blood: mechanical work and energetics Am J Physiol Heart Circ Physiol, November 1, 2009; 297(5): H1736 - H1743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guo and H.-T. Yang Ca2+ removal mechanisms in mouse embryonic stem cell-derived cardiomyocytes Am J Physiol Cell Physiol, January 1, 2009; 297(3): C732 - C741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Gonzalez, F. Beigi, A. V. Treuer, and J. M. Hare Deficient ryanodine receptor S-nitrosylation increases sarcoplasmic reticulum calcium leak and arrhythmogenesis in cardiomyocytes PNAS, December 18, 2007; 104(51): 20612 - 20617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pasek, J. Simurda, and G. Christe The functional role of cardiac T-tubules explored in a model of rat ventricular myocytes Phil Trans R Soc A, May 15, 2006; 364(1842): 1187 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Salas, M. G. Vila-Petroff, R. A. Venosa, and A. Mattiazzi Contractile recovery from acidosis in toad ventricle is independent of intracellular pH and relies upon Ca2+ influx J. Exp. Biol., March 1, 2006; 209(5): 916 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Reuter, C. Pott, J. I. Goldhaber, S. A. Henderson, K. D. Philipson, and R. H.G. Schwinger Na+-Ca2+exchange in the regulation of cardiac excitation-contraction coupling Cardiovasc Res, August 1, 2005; 67(2): 198 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Fowler, J. R. Naz, M. D. Graham, G. Bru-Mercier, S. M. Harrison, and C. H. Orchard Decreased Ca2+ extrusion via Na+/Ca2+ exchange in epicardial left ventricular myocytes during compensated hypertrophy Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2431 - H2438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoshikawa, H. Hagihara, Y. Ohga, C. Nakajima-Takenaka, K.-y. Murata, S. Taniguchi, and M. Takaki Calpain inhibitor-1 protects the rat heart from ischemia-reperfusion injury: analysis by mechanical work and energetics Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1690 - H1698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hagihara, Y. Yoshikawa, Y. Ohga, C. Takenaka, K.-y. Murata, S. Taniguchi, and M. Takaki Na+/Ca2+ exchange inhibition protects the rat heart from ischemia-reperfusion injury by blocking energy-wasting processes Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1699 - H1707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ballard-Croft, D. Carlson, D. L. Maass, and J. W. Horton Burn trauma alters calcium transporter protein expression in the heart J Appl Physiol, October 1, 2004; 97(4): 1470 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Henderson, J. I. Goldhaber, J. M. So, T. Han, C. Motter, A. Ngo, C. Chantawansri, M. R. Ritter, M. Friedlander, D. A. Nicoll, et al. Functional Adult Myocardium in the Absence of Na+-Ca2+ Exchange: Cardiac-Specific Knockout of NCX1 Circ. Res., September 17, 2004; 95(6): 604 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. O'Neill, L. Miller, R. Hinch, and D. A. Eisner Interplay between SERCA and sarcolemmal Ca2+ efflux pathways controls spontaneous release of Ca2+ from the sarcoplasmic reticulum in rat ventricular myocytes J. Physiol., August 15, 2004; 559(1): 121 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chorvatova, G. Hart, and M. Hussain Na+/Ca2+ exchange current (INa/Ca) and sarcoplasmic reticulum Ca2+ release in catecholamine-induced cardiac hypertrophy Cardiovasc Res, February 1, 2004; 61(2): 278 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Baumann, S. Poitry, A. Roatti, and A. J. Baertschi Plasmalemmal KATP channels shape triggered calcium transients in metabolically impaired rat atrial myocytes Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2296 - H2305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Choi, Y. Zhong, B. D. Hoit, I. L. Grupp, H. Hahn, K. W. Dilly, S. Guatimosim, W. J. Lederer, and M. A. Matlib Defective intracellular Ca2+ signaling contributes to cardiomyopathy in Type 1 diabetic rats Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1398 - H1408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yang, C. Pascarel, D.S. Steele, K. Komukai, F. Brette, and C.H. Orchard Na+-Ca2+ Exchange Activity Is Localized in the T-Tubules of Rat Ventricular Myocytes Circ. Res., August 23, 2002; 91(4): 315 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bassani and J. W. M. Bassani Contribution of Ca2+ transporters to relaxation in intact ventricular myocytes from developing rats Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2406 - H2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. TAKIMOTO, A. YAO, H. TOKO, H. TAKANO, M. SHIMOYAMA, M. SONODA, K. WAKIMOTO, T. TAKAHASHI, H. AKAZAWA, M. MIZUKAMI, et al. Sodium calcium exchanger plays a key role in alteration of cardiac function in response to pressure overload FASEB J, March 1, 2002; 16(3): 373 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A.I.P. Trines, C. J. Slager, T. A.M. Onderwater, J. M.J. Lamers, P. D. Verdouw, and R. Krams Oxygen wastage of stunned myocardium in vivo is due to an increased oxygen cost of contractility and a decreased myofibrillar efficiency Cardiovasc Res, July 1, 2001; 51(1): 122 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. SUH, E. T. SHIGENO, J. D. MORROW, B. COX, A. E. ROCHA, B. FREI, and T. M. HAGEN Oxidative stress in the aging rat heart is reversed by dietary supplementation with (R)-{alpha}-lipoic acid FASEB J, March 1, 2001; 15(3): 700 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M.N. Terracciano, K. D. Philipson, and K. T. MacLeod Overexpression of the Na+/Ca2+ exchanger and inhibition of the sarcoplasmic reticulum Ca2+-ATPase in ventricular myocytes from transgenic mice Cardiovasc Res, January 1, 2001; 49(1): 38 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hattori, N. Matsuda, J. Kimura, T. Ishitani, A. Tamada, S. Gando, O. Kemmotsu, and M. Kanno Diminished function and expression of the cardiac Na+-Ca2+ exchanger in diabetic rats: implication in Ca2+ overload J. Physiol., August 15, 2000; 527(1): 85 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. McCaslin and J. Butterworth Bupivacaine Suppresses [Ca2+]i Oscillations in Neonatal Rat Cardiomyocytes with Increased Extracellular K+ and Is Reversed with Increased Extracellular Mg2+ Anesth. Analg., July 1, 2000; 91(1): 82 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Egger and E. Niggli Paradoxical block of the Na+-Ca2+ exchanger by extracellular protons in guinea-pig ventricular myocytes J. Physiol., March 1, 2000; 523(2): 353 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A W Trafford, M E Diaz, G C Sibbring, and D A Eisner Modulation of CICR has no maintained effect on systolic Ca2+: simultaneous measurements of sarcoplasmic reticulum and sarcolemmal Ca2+ fluxes in rat ventricular myocytes J. Physiol., January 15, 2000; 522(2): 259 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Harwood, F. Howarth, J. Altringham, and E White Rate-dependent changes in cell shortening, intracellular Ca(2+) levels and membrane potential in single, isolated rainbow trout (Oncorhynchus mykiss) ventricular myocytes J. Exp. Biol., January 2, 2000; 203(3): 493 - 504. [Abstract] [PDF]
|
|
|
|
|
|
B. Pieske, L. S. Maier, D. M. Bers, and G. Hasenfuss Ca2+ Handling and Sarcoplasmic Reticulum Ca2+ Content in Isolated Failing and Nonfailing Human Myocardium Circ. Res., July 9, 1999; 85(1): 38 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dipla, J. A. Mattiello, K. B. Margulies, V. Jeevanandam, and S. R. Houser The Sarcoplasmic Reticulum and the Na+/Ca2+ Exchanger Both Contribute to the Ca2+ Transient of Failing Human Ventricular Myocytes Circ. Res., March 5, 1999; 84(4): 435 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Choi and D A Eisner The role of sarcolemmal Ca2+-ATPase in the regulation of resting calcium concentration in rat ventricular myocytes J. Physiol., February 15, 1999; 515(1): 109 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, G. Chu, E. G. Kranias, and D. M. Bers Cardiac myocyte calcium transport in phospholamban knockout mouse: relaxation and endogenous CaMKII effects Am J Physiol Heart Circ Physiol, April 1, 1998; 274(4): H1335 - H1347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C L Overend, S C O'Neill, and D A Eisner The effect of tetracaine on stimulated contractions, sarcoplasmic reticulum Ca2+ content and membrane current in isolated rat ventricular myocytes J. Physiol., March 15, 1998; 507(3): 759 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yao, H. Matsui, K. W. Spitzer, J. H. B. Bridge, and W. H. Barry Sarcoplasmic reticulum and Na+/Ca2+ exchanger function during early and late relaxation in ventricular myocytes Am J Physiol Heart Circ Physiol, December 1, 1997; 273(6): H2765 - H2773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y Antipenko and M. A Kirchberger Membrane phosphorylation protects the cardiac sarcoplasmic reticulum Ca2+-ATPase against chlorinated oxidants in vitro Cardiovasc Res, October 1, 1997; 36(1): 67 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Antipenko, A. I. Spielman, and M. A. Kirchberger Comparison of the Effects of Phospholamban and Jasmone on the Calcium Pump of Cardiac Sarcoplasmic Reticulum. EVIDENCE FOR MODULATION BY PHOSPHOLAMBAN OF BOTH Ca2+ AFFINITY AND Vmax(Ca) OF CALCIUM TRANSPORT J. Biol. Chem., January 31, 1997; 272(5): 2852 - 2860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.E. Diaz, S.J. Cook, J.P. Chamunorwa, A.W. Trafford, M.K. Lancaster, S.C. O'Neill, and D.A. Eisner Variability of Spontaneous Ca2+ Release Between Different Rat Ventricular Myocytes Is Correlated With Na+-Ca2+ Exchange and [Na+]i Circ. Res., May 1, 1996; 78(5): 857 - 862. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Bouchard, R. B. Clark, and W. R. Giles Effects of Action Potential Duration on Excitation-Contraction Coupling in Rat Ventricular Myocytes : Action Potential Voltage-Clamp Measurements Circ. Res., May 1, 1995; 76(5): 790 - 801. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Ji, M. J. Lalli, G. J. Babu, Y. Xu, D. L. Kirkpatrick, L. H. Liu, N. Chiamvimonvat, R. A. Walsh, G. E. Shull, and M. Periasamy Disruption of a Single Copy of the SERCA2 Gene Results in Altered Ca2+ Homeostasis and Cardiomyocyte Function J. Biol. Chem., November 22, 2000; 275(48): 38073 - 38080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bassani and J. W. M. Bassani Contribution of Ca2+ transporters to relaxation in intact ventricular myocytes from developing rats Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2406 - H2413. [Abstract] [Full Text] [PDF]
|
|