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Cardiovascular Research 1983 17(2):70-74; doi:10.1093/cvr/17.2.70
© 1983 by European Society of Cardiology
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Copyright © 1983, European Society of Cardiology

A study of cold cardioplegic myocardial protection in rats: an experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury

C G A McGREGOR*, J HANNAN, A F SMITH, A L MUIR and D J WHEATLEY{dagger}

From the Departments of Clinical Surgery, Medical Physics, Clinical Chemistry and Medicine, University of Edinburgh, Royal Infirmary, Edinburgh

* Present address and address for reprints: Department of Cardiothoracic Surgery, Papworth Hospital, Papworth Everard, Cambridge CB3 8RE.

{dagger} Present address: Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, Glasgow.

This study compares the use of St Thomas' cardioplegia and cold saline cardioplegia at 4°C for protection against global myocardial ischaemia in a model of heterotopic cardiac transplantation in rats of isogeneic strain. The parameters of myocardial injury applied were the uptake of Technetium 99m pyrophosphate (Tc 99m Pyp) in the transplanted heart and the measurement of serum total creatine kinase (CK) and creatine kinase MB (CK:MB) isoenzyme activity. The findings indicate that St Thomas' cardioplegic solution confers improved myocardial protection compared with normal saline as judged by statistically significant differences between: (a) the uptake of Tc 99m Pyp between the two solutions at 30 min storage: and (b) the peak total serum creatine kinase enzyme activity at 90 min storage. Excellent correlation was present between the uptake of Tc 99m Pyp and peak total serum creatine kinase activity. Further application of this model is described.

KEYWORDS cardioplegia; myocardium (rat); Tc 99m pyrophosphate; enzymes


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